Spectrophotometric Determination of Blood pH
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چکیده
منابع مشابه
Spectrophotometric determination of blood oxygen.
Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human ...
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The use of the spectrophotometer as a means of estimating the relative and total concentrations of blood pigments was first introduced by Hiifner (1, 2), following the theoretical work of Vierordt (3). This author published tables whereby the relative concentrations of oxyhemoglobin, hemoglobin, carboxyhemoglobin, and methemoglobin could be estimated from the quantitative character of the absor...
متن کاملSpectrophotometric determination of the oxygen saturation of whole blood.
A new approach to the spectrophotometric determination of blood oxygen saturation has been made which is based on the absorption maximum of oxyhemoglobin and the isosbestic point of oxyhemoglobin and hemoglobin. The precision of this new method is comparable to that of the manometric procedure in which the Van Slyke-Neil1 apparatus is employed; there is also good correlation between results obt...
متن کاملThe spectrophotometric determination of the oxygen saturation of blood.
ORDY AND DRABKIN (1) have described a simplified spectrophotometric procedure for the determination of oxygen saturation of whole hemolyzed blood. The described method of sampling by use of tonometers, although technically precise, is somewhat inconvenient for routine clinical use. In a note appended to the article, Marsh described a simpler modification. He collects the samples in oiled, hepar...
متن کاملDetermination of the carboxyhaemoglobin saturation of blood by spectrophotometric analysis.
The spectrophotometric method of Commins and Lawther (I965) for the determination of carboxy-haemoglobin saturation in blood was found to lead to systematic undervaluation, because of dissociation of the carboxyhaemoglobin, and physical dissolution of the carbon monoxide in the solvent used for the analysis. The theoretical relationship between the true and apparent carboxyhaemoglobin saturatio...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64217-6